Production of rice inoculated with diazotrophs marked with induced resistance to the antibiotic streptomycin

Abstract

The objective of this study was to evaluate the effects of inoculation with Herbaspirillum spp. and Burkholderia spp. in relation to yield components of rice. Two experiments were conducted: the first, in the greenhouse, and the second, under field conditions, with two rice cultivars (IR42 and IAC4440). In the experiment in a greenhouse, for cv. IR42, and the ZAE94 ZALIR42 strains (Herbaspirillum seropedicae), and M130 and M171 (Burkholderia spp.) Were used. In 'IAC4440: ZAE94 and Z3 (H. seropedicae), M130 and M209 (. Burkholderia spp), with four replications. As a control, non-inoculated plants were used. The bacteria were checked for resistance to the antibiotic streptomycin, and Herbaspirillum spp. showed resistance to 100 mg kg-1 streptomycin as Burkholderia spp. to 150 mg kg-1. The seed inoculation was performed by pelleting with peat containing the bacteria multiplied in the midst Dygs. Four seeds per pot, and held up a collection at the end of the cycle cultivars were planted. In the field, samples were collected according to the phenological stage of the cultivars, evaluating: population of diazotrophic bacteria in plants, dry matter, total N of shoots and grain production. In the greenhouse, in general, inoculation had a positive effect, varying with each strain and cultivar used. In the field, the most promising results pointed to the IAC4440 grow in increments of up to 54% in grain yield due to inoculation with diazotrophs.