Inhibition of Corynespora cassiicola With Extracts and Fractions of Exotic and Amazonian Plants

Socorro Cristina  Tavares Costa; Antônia Queiroz Lima  Queiroz Lima Souza; Ana Francisca Tibúrcia Amorim Ferreira e Ferreira; Jania Lília  Silva Bentes

Socorro Cristina Tavares Costa Universidade Federal do Amazonas https://orcid.org/0000-0003-3146-7028
Antônia Queiroz Lima de Souza Universidade Federal do Amazonas https://orcid.org/0000-0001-5602-8617
Ana Francisca T. A. F. e Ferreira Universidade Federal do Amazonas https://orcid.org/0000-0002-7484-8106
Jania Lília Silva Bentes Universidade Federal do Amazonas https://orcid.org/0000-0001-7302-5661

Keywords: Solanum lycopersicum, target spot disease, alternative management

Abstract

The control of the disease by plant extracts is a financially and ecologically feasible alternative for tomato small holders in Amazonas. Extracts and fractions of P. sprucei, C. cajucara, P. marginatum and C. zedoaria were evaluated in vitro and in vivo on tomato plants against the pathogen C. Cassiicola pathogen. The aqueous plant extracts at concentrations of 0%, 10%, 20%, 30%, 40% and 50%, and the effect of the hexanic, ethyl acetate and methanolic fractions, were evaluated on mycelial growth, sporulation and conidium germination of the fungus. Direct bioautography was performed and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined. Aqueous extracts of P. marginatum were used to evaluate the reduction of disease symptoms in tomato seedlings. The hexane and ethyl acetate fractions of P. marginatum reduced mycelial growth, spore formation and conidium germination. In direct bioautography, zones of mycelial growth inhibition were observed in the hexane and ethyl acetate fractions of P. marginatum, with MIC of 2.5 mg mL-1. P. marginatum extract reduced disease severity in tomato seedlings, but was not effective in controlling the pathogen as a single management procedure.

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Author Biographies

Socorro Cristina Tavares Costa, Universidade Federal do Amazonas

Agronomist graduated at UFAM. Master degreee Tropical Agronomy Pos-Graduate Program at Federal University of Amazonas- UFAM

Antônia Queiroz Lima de Souza, Universidade Federal do Amazonas

Biologista at UFAM (1996), MSc (2001) and PhG ( 2006) on Genetic and Evolutin at Federal University os São Carlos ( UFSCar). Professor of Microbiology and Genetics at Agrarian Faculty- UFAM

Ana Francisca T. A. F. e Ferreira, Universidade Federal do Amazonas

Agronomis ( UFAM). MSc on Tropical Agronony ( PPGATR-UFAM). PhG on Phytopthology (UFV). Pos-Doc Research at Agrarian Faculty- UFAM

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