USE OF bromophenol blue KEPT A 4A EC 29 ° C, AS VITAL STAINING METHOD FOR EVALUATION OF SPERM OVINOv
Abstract
To assess the morphology of espermatoz6ide sheep, semen smears (n = 320) were stained with bromophenol blue (ABR = 160) and eosin-nigrosin (DE = 160). The dyes were stored at two different temperatures: 4 ° C and 29 ° C (room temperature) and used ap6s 30 days of storage. By observation by microscopy three types of spermatozoa were identified: live sperm, which showed no staining, dead sperm with acrosome reacted completely stained purple (EN) or completely blue (ABR); purple spermatozoa with an intact acrosome (acrosome white) or colored in blue with intact acrosome (acrosome white). The comparison between the two dyes showed that both are efficient to evaluate the percentage of live and dead cells with the following percentages: EN4 ° C = 86.60; EN29 ° C = 86.30; ABR4 ° C and ABR29 = 84.27 C = 86.70 °. Both colora95es were also efficient evaluation of sperm pathologies, with no significant difference between them, regardless of storage temperature. However, bromophenol blue was more efficient at ambient temperature. The slides stained with bromophenol blue had better viewing under the microscope no particles which may cause artifacts which hamper the evaluation of sperm cell, which allows the use in field tests for andrological sheep.Downloads
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