SEEDING IN VITRO AND SEEDLING ACCLIMATIZATION star fruit
Abstract
The germination behavior in vitro is variable depending on each species. Therefore, this work aims to verify how this process occurs, and defines a methodology for acclimatization of plantlets obtained in vitro star fruit. The experiment was conducted at the Laboratory of Tissue Culture, Faculty of Agriculture and Veterinary Sciences (FCAV) UNESP, Jaboticabal (SP). Seeds from ripe fruits of cultivar B-10 belonging to the orchard FCAV / UNESP were decontaminated with 70% alcohol and hypochlorite 30% (w / v) and germinated amid MS1 / 2, with and without addition of 2 mL .L-1 anticontaminante ppm. Plantlets in vitro were tested for acclimatization in two seasons, with 1-2 days (3.0 cm) and 5-6 days (4.5 cm) after seed germination. It was found that in vitro seeding procedure is feasible to obtain star fruit seedlings and seedlings with 1-2 days after germinaçãoa do not survive in vitro, while seedlings of 5-6 days after germination showed 98% survival during acclimatization.Downloads
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