Selection of protein extraction method for electrophoretic analysis of seeds of the Physic nut genus

Abstract

Abstract: The study aimed to partially extract and purify the proteins present in the seed of Jatropha curcas L., select the best extraction method to characterize the protein electrophoretic profile of genotypes belonging to the Center for Genetic Improvement and Biotechnology, prioritizing ribosome-inactivating proteins (RIPs). The standardization was conducted in four ways, which distinguished in the form of protein extraction and in the extraction solutions, which are: I) Sodium Acetate Buffer (0.45 M) and II), HCl (0.1%)/NaCl (0.6 M), III) NaOH (1 M) and IV) Phosphate-saline buffer (PBS). The method of protein extraction with the best electrophoretic resolution, which was with PBS, using albumen from the stored seeds. The protein concentrations of the samples ranged from 2.75 to 5.15 mg / mL between the genotypes but did not differ statistically from each other by the Tukey test at 5% probability. It was possible to identify RIPs through the electrophoretic profile, with a molecular weight of 28 kDa, in all evaluated genotypes. Profiles from different bands were observed in the UFRB05 and UFRB15 genotypes. The UFRB05 genotype stood out when associated with the quality of stored seeds and oil content.