Histological and ultrastructural analysis of the Banana cv. Prata-Anã embryogenic calluses and cell suspension

  • MARLÚCIA SOUZA PÁDUA Universidade Federal de Lavras
  • CAROLINA DELFIM LIMA Universidade Federal de Lavras
  • LUCIANO VILELA PAIVA Universidade Federal de Lavras
  • DOUGLAS BARDUCHE Universidade Federal de Lavras
  • BRENO RÉGIS SANTOS Universidade Federal de Alfenas
  • VANESSA CRISTINA STEIN Universidade Federal de Goiás

Resumo

 The analysis of the developmental stages during somatic embryogenesis has been essential to elucidate the in vitro plants embryogenesis process and can validate the somatic embryogenesis induction and progression. This work aimed to characterize the embryogenic calluses and cell suspension of Banana cv. Prata-Anã, anatomically and ultrastructurally, respectively. For the calluses induction and anatomical analyses, the male flower were disinfested and inoculated on MA1 solid medium. At intervals of 30 days up to 11 months, calluses were collected, fixed in FAA and prepared for analysis in optical microscopy. For the cell suspension and ultrastructural analyses, calluses4 months old calluses, obtained on the first experiment, were transferred to different liquids cultures mediums: MRT, MGM, CNPMF or MA2 and maintained in aggitation. At intervals of 30 days calluses were collected and prepared for analysis in scanning electron microscope. The male flowers showed, after three months on culture medium, heterogeneous calluses formed by clusters of small isodiametric cells that gave rise to globular structure with meristems tissues, clearly differentiated from other cells around the calluses. The globular structure, after ten months on the culture medium, developed to torpedo stage showing the meristematic tissues protoderm, ground meristem and procambium. The cell suspension cultivated on MRT culture medium showed embryos on globular and torpedo stage.

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Biografia do Autor

MARLÚCIA SOUZA PÁDUA, Universidade Federal de Lavras
Doutoranda em Biotecnologia Vegetal
CAROLINA DELFIM LIMA, Universidade Federal de Lavras
Mestre em Fisiologia Vegetal
LUCIANO VILELA PAIVA, Universidade Federal de Lavras
Professor Associado. Coordenador do Programa de Pós-graduação em Biotecnologia Vegetal
DOUGLAS BARDUCHE, Universidade Federal de Lavras
Doutorando em Fitotecnia
BRENO RÉGIS SANTOS, Universidade Federal de Alfenas
Professor Associado
VANESSA CRISTINA STEIN, Universidade Federal de Goiás
Regional Jataí - Curso de Ciências Biológicas

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Publicado
2015-08-25
Seção
Artigos Científicos